anti mouse antibody blocker  (Biosynth Carbosynth)

Journal: PLoS ONE

Article Title: Mechanism of Fcγ Receptor-Mediated Trogocytosis-Based False-Positive Results in Flow Cytometry

doi: 10.1371/journal.pone.0052918

Figure Lengend Snippet: ( A ) Effects of HAMA blockade on FcγR-mediated trogocytosis (n = 10). Heparinized whole blood samples were added with or without 1 µl of HAMA inhibitor, TRU block, and then made to react with the PE-labeled anti-CD8α (HIT8a) and FITC-labeled anti-CD15 (H198) Abs. After depletion of erythrocytes, these cells were subjected to FCM. PE-labeled mouse IgG1 and FITC-labeled mouse IgM were used as isotype-matched controls for HIT8a and H198, respectively. Student t -test for paired samples was applied for statistical analysis. ( B ) Difference in the inhibition rates of FcγR-mediated trogocytosis by the HAMA inhibitor between HAMA high sera and HAMA low sera. The serum samples were divided into 2 groups, including HAMA high serum (more than 10 ng/ml, n = 5) and HAMA low serum (less than 10 ng/ml, n = 5). The decrease rates of CD8 + granulocytes by treatment with TRU block were calculated from the data presented in . Student t -test for unpaired samples was applied for statistical analysis.

Article Snippet: Next, whole blood samples were added with or without 1 μl of HAMA inhibitor, TRU block (Meridian Life Science, Saco, ME), and then made to react with 0.1 μg of the PE-labeled anti-CD8α and FITC-labeled anti-CD15 Abs for 20 min at room temperature.

Techniques: Blocking Assay, Labeling, Inhibition